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Scapy is a powerful interactive packet manipulation program.
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Nipper - Neat Internet Protocol Packet EditoR - is a graphical packet generation tool.
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Ostinato is a packet crafter and traffic generator for network engineers. Wireshark in Reverse!
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packeth is GUI and CLI packet generator tool for ethernet.
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PacketSled is next generation network forensics and breach detection.
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Bit-Twist is a powerful packet generator (10Gbps on single core) and editor, written in POSIX-compliant C. Designed to complement tcpdump, it can replay captured traffic from pcap files onto live networks and supports Windows, Linux, BSD, and macOS.