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UGENE - UGENE is free open-source cross-platform bioinformatics software
BioEdit - Biological sequence alignment editor
Chromas - Two software applications are available from Technelysium: Chromas and ChromasPro.
DNADynamo - DNA analysis, cloning and sequencing software
ApE - A plasmid Editor - Software for viewing, editing and analyzing plasmid sequences.
SnapGene Viewer - SnapGene offers the fastest and easiest way to plan, visualize, and document DNA cloning and PCR. You can easily annotate features and design primers.