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Apache JMeter - Apache JMeter™.
Fast.com - Quickly test your internet speed with this fast-loading speed test powered by Netflix.
locust - An open source load testing tool written in Python.
LibreSpeed - Self-hosted Speedtest for HTML5. Easy setup, examples, configurable, mobile friendly.
Selenium - Selenium automates browsers. That's it! What you do with that power is entirely up to you. Primarily, it is for automating web applications for testing purposes, but is certainly not limited to just that.
SpeedOf.Me - SpeedOf.Me is an HTML5 Internet speed test. No Flash or Java needed!