Aseprite - Aseprite is an art program dedicated to the creation of pixel art.
CSS Sprite Generator - A tool for generating image sprites and CSS for your web site.
Pixel Art Studio - A purpose-built drawing tool for pixel artists
Sprite Cow - Sprite Cow helps you get the background-position, width and height of sprites within a spritesheet...
Pixilart - A drawing app for delightfully retro pixel art
SpritePad - The easiest way to create your CSS sprites