Aseprite - Aseprite is an art program dedicated to the creation of pixel art.
CSS Sprite Generator - A tool for generating image sprites and CSS for your web site.
Pixel Art Studio - A purpose-built drawing tool for pixel artists
SpritePad - The easiest way to create your CSS sprites
Pixilart - A drawing app for delightfully retro pixel art
SpriteSheep - SpriteSheep lets you easily and visually generate CSS sprite sheets and CSS code with all sprites...