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Aseprite - Aseprite is an art program dedicated to the creation of pixel art.
Spine - Spine is 2D animation software designed specifically for software and game development.
Grafx2 - GrafX2 is a bitmap paint program inspired by the Amiga programs Deluxe Paint and Brilliance.
Synfig Studio - Just another WordPress site
LibreSprite - Free and open source program to create animated sprites.
Live2D Cubism - Live2D is the world's first drawing technology to enable 3D rendering of 2D images.