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Aseprite - Aseprite is an art program dedicated to the creation of pixel art.
CSS Sprite Generator - A tool for generating image sprites and CSS for your web site.
Pixel Art to CSS - Draw and animate pixel art, export the results to CSS
Sprite Cow - Sprite Cow helps you get the background-position, width and height of sprites within a spritesheet...
Pixel Art Studio - A purpose-built drawing tool for pixel artists
SpritePad - The easiest way to create your CSS sprites