Pixel Art Studio VS SpriteSheep

Aseprite - Aseprite is an art program dedicated to the creation of pixel art.

CSS Sprite Generator - A tool for generating image sprites and CSS for your web site.

Pixel Studio - The Pixel Art Is Always Fun

Sprite Cow - Sprite Cow helps you get the background-position, width and height of sprites within a spritesheet...

Pixel Art to CSS - Draw and animate pixel art, export the results to CSS

SpritePad - The easiest way to create your CSS sprites