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SnapGene Viewer - SnapGene offers the fastest and easiest way to plan, visualize, and document DNA cloning and PCR. You can easily annotate features and design primers.
Gene Construction Kit - Molecular biology software for plasmid mapping, DNA sequence editing, identifying open reading frames (ORF's), restriction enzyme site analysis and PCR primer design.
Open Vector Editor Electron - A simple file based genbank/fasta file viewer and editor. Works with mac/windows/linux
UGENE - UGENE is free open-source cross-platform bioinformatics software
Mendelgen - Plasmid editing simplified. Quickly optimize codons, align sequences, simulate digestions, and more.
DNADynamo - DNA analysis, cloning and sequencing software