There are several DNA analysis apps. But most of them are subscription-based. DNADynamo is a static license app. It means that once you purchase a license, you get full support for these licenses, including future updates. We have five licenses in my lab, and I can easily transfer the licence on the computer of the finished student to the one who has just started. The interface is improving with every update, and I can say I love the app!
SnapGene Viewer - SnapGene offers the fastest and easiest way to plan, visualize, and document DNA cloning and PCR. You can easily annotate features and design primers.
FamLinkX - FamLink homep
BioEdit - Biological sequence alignment editor
Serial Cloner - Serial Cloner is a Molecular Biology software.
UGENE - UGENE is free open-source cross-platform bioinformatics software
Genome Compiler - Genome Compiler is an all-in-one free software platform for biologists. You can intuitively visualize & design DNA, import, manage and share your data.